Evaluation of a rapid Cryptosporidium/Giardia immunochromatographic test for diagnosis of giardiasis in dogs
نویسندگان
چکیده
The aim of this study was to evaluate the performance of the RIDA® Quick Cryptosporidium/Giardia-Combi test (R-Biopharm AG) as a rapid method for the routine diagnosis of giardiasis in dogs. Compared to an ELISA coproantigen test (Prospect® Giardia Microplate Assay)), the immunochromatographic test had a sensitivity of 83 %, a specificity of 79 %, a positive predictive value of 33 %, and a negative predictive value of 97 %. The k value was 0.5. Although the immunochromatographic test is simpler to perform, it can not replace the Giardia ELISA coproantigen test for rapid diagnosis of giardiasis in dogs, since its sensitivity and specificity appear to be not comparable to those of the ELISA kit. No Cryptosporidium infection was detected.
منابع مشابه
Diagnosis of Giardia and Cryptosporidium Infection in Children with Diarrheal Illness Using Rapid Immunochromato- graphic Assay
Background: Giardia and Cryptosporidium are both enteric pathogens causing diarrhea with no reliable method for their diagnosis. The aim of our study was to evaluate the diagnostic performance of a rapid immunochromatographic (IC) ImmunoCard STAT! Crypt/Giardia assay (Meridian Bioscience, Inc.) in comparison to composite reference standard (CRS) of both wet mount microscopy and ELISA copro-anti...
متن کاملEvaluation of the new ImmunoCard STAT! CGE test for the diagnosis of Amebiasis Évaluation d’un nouveau test ImmunoCard STAT ! CGE pour le diagnostic de l’amibiase
For many years, microscopic examination of stool samples has been considered to be the “gold standard” for diagnosis of intestinal parasites although the Polymerase Chain Reaction (PCR) analysis is increasingly utilized due to its high accuracy. Recently, PCR has been approved by the World Health Organization as the current method of choice for the diagnosis of Entamoeba histolytica infection. ...
متن کاملEvaluation of an Immunoassay-Based Algorithm for Screening and Identification of Giardia and Cryptosporidium Antigens in Human Faecal Specimens from Saudi Arabia
An immunoassay-based algorithm, involving three commercial kits, was introduced and evaluated for screening and identification of Giardia/Cryptosporidium antigens in human stool specimens. Initially, Giardia/Cryptosporidium Chek kit (TechLab), an enzyme-linked immunosorbent assay (ELISA), was adopted for screening. The ELISA-positive reactions were subsequently characterised by RIDA Quick Giard...
متن کاملCommercial assay for detection of Giardia lamblia and Cryptosporidium parvum antigens in human fecal specimens by rapid solid-phase qualitative immunochromatography.
The ImmunoCard STAT! Cryptosporidium/Giardia rapid assay (Meridian Bioscience, Inc.) is a solid-phase qualitative immunochromatographic assay that detects and distinguishes between Giardia lamblia and Cryptosporidium parvum in aqueous extracts of human fecal specimens (fresh, frozen, unfixed, or fixed in 5 or 10% formalin or sodium acetate-acetic acid-formalin). By using specific antibodies, an...
متن کاملEvaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens.
There is an increasing demand for diagnostic testing for Giardia intestinalis (G. lamblia) and Cryptosporidium parvum, with a priority being placed on obtaining diagnostic results in an efficient and timely manner. Several commercial companies have developed rapid diagnostic tests that are simple to perform and can be completed in less time than traditional methods for detecting Giardia and Cry...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006